Cytoscape transcription factor4/20/2023 2 MATERIALS AND METHODS 2.1 RNA isolation, labeling, and microarray hybridization In recent years, studies have found that some lncRNAs, such as HOTAIR, 10 MALAT1, 11 PVT1, 12 H19, 13 and XIST, 14 play important regulatory roles in cell proliferation, invasion, and migration in cervical cancer. 5- 8 The versatility of the lncRNA structure enables it to bind to target molecules and participate in the regulation of gene expression in a variety of ways at the epigenetic, transcriptional, and posttranscriptional levels, contributing to the occurrence and development of various human diseases, especially apoptosis, invasion, and metastasis of various tumors. ![]() Approximately 76% of the human genome is transcribed as lncRNA, which is longer than 200 nucleotides and has a structure more complex than mRNAs. The search for effective markers is of great significance for perfecting molecular subtype classification and molecular targeted therapy for cervical cancer. Molecular targeted therapy provides new ideas for the treatment of these patients. The 5-year survival rate of patients with stage IV disease is less than 15%. Therefore, the efficacy of traditional treatment for patients with the advanced and relapsed disease is unsatisfactory. 4 However, advanced patients have no opportunity for surgery, the lesion may not be removed completely for those with lymph node metastasis, and relapsed and uncontrolled patients face the problem of drug resistance. For patients with FIGO staging of IB to IIA without lymph node metastasis, the five-year survival rate can reach 88%–95%. 3 The traditional treatment for cervical cancer is surgery combined with radiotherapy, chemotherapy or radical radiotherapy. 2 According to the American Cancer Society, there are 14,480 new diagnoses and more than 4290 deaths from cervical cancer in 2021 in the USA. 1 Its annual rate is 16.56 per 100,000 people, and the mortality rate is as high as 5.04 per 100,000 people. 1 Both the incidence and mortality rates of cervical cancer are ranked fourth among malignancies in women. Globally, there were 604,127 new cases and 341,831 deaths from cervical cancer in 2020. The lncRNA-TF regulatory network plays an important role in the occurrence and progression of cervical cancer, and E2F4 may be a critical transcription factor in the regulatory network.Īlthough the level of cervical cancer screening and HPV infection prevention and control is gradually being emphasized and improved, cervical cancer still has high morbidity and high mortality, and is still the most common gynecological cancer worldwide. The lncRNA-trans-regulation network analysis suggested that E2F4 may be the core transcription factor in the lncRNA-TF regulatory network in cervical cancer. Through cis-regulation analysis of DELs, the cis-regulatory relationship of 96 DELs was predicted. Resultsįunction prediction of DELs (both up-regulated and down-regulated) shows that the highest frequency Cellular Component (CC) item is cytosol, the highest frequency Molecular function (MF) item is mitotic cell cycle and the highest frequency Biological Process (BP) item is protein binding. Functional prediction of DELs was then performed and their cis-regulatory and trans-regulatory networks were explored. ![]() For DEMs, functional annotation and pathway analysis were performed using DAVID. Second, differentially expressed mRNAs (DEMs) and differentially expressed lncRNAs (DELs) (|fold change| ≥ 2 and p < 0.05) between the two groups were screened. Methodsįirst, three paired tissues (cancer tissues and noncancerous tissues) from patients with cervical squamous cell carcinoma were collected, grouped, and analyzed by microarray. Therefore, there is a need to continue studying the mechanism of cervical cancer to explore effective gene targets and precise targeted therapy drugs. Despite improvements in therapeutic strategies, the network regulation mechanism remains unclear and the treatment effect is not satisfactory. Cervical cancer is the most common gynecological cancer worldwide and is associated with high morbidity and mortality.
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